Influence of Activated Carbon–Remediated Olive Mill Wastewater on Germination and Early Growth of Barley and Fenugreek

Authors

  • Kenza Kadi Biotechnology, Water, Environment and Health Laboratory, Abbes Laghrour University of Khenchela, Khenchela, Algeria; Faculty of Nature and Life Sciences, Abbes Laghrour University of Khenchela, Khenchela, Algeria https://orcid.org/0000-0003-2866-635X
  • Zakia Gueboudji Biotechnology, Water, Environment and Health Laboratory, Abbes Laghrour University of Khenchela, Khenchela, Algeria; Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, Mostefa Ben Boulaid Batna 2 University, Batna, Algeria
  • Dalila Addad Biotechnology, Water, Environment and Health Laboratory, Abbes Laghrour University of Khenchela, Khenchela, Algeria; RNAMS Laboratory, Larbi ben M’Hidi University, Oum El Bouaghi, Algeria
  • Sofia Hamli Biotechnology, Water, Environment and Health Laboratory, Abbes Laghrour University of Khenchela, Khenchela, Algeria; Faculty of Nature and Life Sciences, Abbes Laghrour University of Khenchela, Khenchela, Algeria
  • Nawal Mekersi Biotechnology, Water, Environment and Health Laboratory, Abbes Laghrour University of Khenchela, Khenchela, Algeria; Faculty of Nature and Life Sciences, Abbes Laghrour University of Khenchela, Khenchela, Algeria
  • Nadjwa Aissani Biotechnology, Water, Environment and Health Laboratory, Abbes Laghrour University of Khenchela, Khenchela, Algeria; Faculty of Nature and Life Sciences, Abbes Laghrour University of Khenchela, Khenchela, Algeria
  • Nehal Shaarawy Forestry and Timber Trees Department, Horticulture Research Institute, Agricultural Research Center, 9 El-Gamaa street, Giza 12619, Egypt
  • Alaa S. Marzouk Phytotoxicity Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center 7 Nadi El-Seid Street, Dokki, Giza 12618, Egypt
  • Youssef Dewer Phytotoxicity Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center 7 Nadi El-Seid Street, Dokki, Giza 12618, Egypt
  • Anis Ahmad Chaudhary Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia
  • Walid Elfalleh Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia
  • Feras Afifi Al-Leith University College, Umm Al-Qura University, 24382, Makkah, Saudi Arabia
  • Hamdi Bendif Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia

Keywords:

Olive mill wastewater, Treatment, Infiltration-percolation, Olive pomace, Seed germination

Abstract

The disposal of olive mill wastewater (OMW) poses significant environmental challenges because of its high organic load and phytotoxic compounds. This study focused on treating OMW from a modern oil mill using a filtration-percolation technique and evaluated its impact on the germination of barley and fenugreek seeds. Filters were prepared using lime and charcoal derived from the mill’s pomace. Four distinct treatments were applied: (1) raw OMW (T0), (2) a lime layer (1.0 cm) sandwiched between two layers of sand (T1), (3) an activated charcoal layer (1.0 cm) derived from pomace sandwiched between two layers of sand (T2), and (4) a combination of lime and activated charcoal derived from pomace, sandwiched between two layers of sand (T3). Extensive physicochemical analyses, including pH, electrical conductivity (EC), total suspended solids (TSS), moisture content (MC), dry matter (DM), total Kjeldahl nitrogen (TKN), biochemical oxygen demand (BOD5), phosphorus (P), polyphenol (PP), and exchangeable cation content (Ca, Na, and K) were performed. The results revealed significant differences between treatments, with substantial reductions in polyphenols, EC, organic matter (OM), total organic carbon (TOC), and BOD5. Treatments T2 and T3 were the most effective depollution of OMW, and T3 exhibited the highest germination rate. These findings highlight the potential of this filtration technique for treating OMW and promoting its reuse in sustainable agriculture.

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Published

2026-10-14

How to Cite

Kadi, K., Gueboudji, Z., Addad, D., Hamli, S., Mekersi, N., Aissani, N., … Bendif, H. (2026). Influence of Activated Carbon–Remediated Olive Mill Wastewater on Germination and Early Growth of Barley and Fenugreek. BioResources, 21(4), 11603–11621. Retrieved from https://ojs.bioresources.com/index.php/BRJ/article/view/25678

Issue

Section

Research Article or Brief Communication