Antioxidant Activity and Stability of Purified Flavonoid Fractions from Rosa davurica Pall

Libu Niu; Jinping Zheng; Ruijian Wang; Yanqiu Liu

Authors

Libu Niu Forestry College of Beihua University of China, Jilin 132013, China https://orcid.org/0009-0007-6222-6231
Jinping Zheng Forestry College of Beihua University of China, Jilin 132013, China https://orcid.org/0009-0005-9856-0085
Ruijian Wang Engineering Research Center of Forestry Biotechnology of Jilin Province of China, Jilin 132013, China https://orcid.org/0009-0001-2264-2966
Yanqiu Liu Forestry College of Beihua University of China, Jilin 132013, China; Engineering Research Center of Forestry Biotechnology of Jilin Province of China, Jilin 132013, China https://orcid.org/0009-0008-5293-2033

Keywords:

Total flavonoids of Rosa davurica Pall, Separation and purification, Ingredient, Antioxidant activity, Stability

Abstract

An ultrasound-assisted deep eutectic solvent was used to extract flavonoids from Rosa davurica Pall., followed by purification on macroporous resin; the separation and purification conditions were optimized. A total of 26 different flavonoid components were identified using ultraviolet spectroscopy, infrared spectroscopy, and ultra-high performance liquid chromatography-mass spectrometry. The antioxidant activity of purified flavonoids was significantly improved, as it not only exhibited excellent scavenging efficiency against DPPH radicals,·OH radicals, O^2- radicals, and ABTS⁺ radicals, but also possessed strong total reducing power. Moreover, its antioxidant activity showed a clear positive correlation with concentration. This study provided a scientific basis for the comprehensive utilization of Rosa davurica Pall.

Antioxidant Activity and Stability of Purified Flavonoid Fractions from Rosa davurica Pall

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