Extracts from Some Tree Pruning Residues and their Cytotoxicity on the HepG2 Cell Line Using the Sulforhodamine-B Assay and their Antifungal Activity

Authors

  • Mohamed Zidan Mohamed Salem Forestry and Wood Technology Department, Faculty of Agriculture (El Shatby), Alexandria University, Alexandria 21545, Egypt https://orcid.org/0000-0002-3961-7935
  • Ahmed K. Al-Kubeisi College of Pharmacy, University of Al Maarif, Al Anbar, 31001, Iraq https://orcid.org/0009-0002-5594-8272
  • Abeer M. Ashmawy Cancer Biology Department, Medical Biochemistry Unit, National Cancer Institute, Cairo University
  • Maisa M. A. Mansour Restoration Department, Faculty of Archaeology, Cairo University, Giza 12613, Egypt https://orcid.org/0000-0002-6983-4789
  • Salma Aboelela Electron Microscope Unit, Cancer Biology Department, National Cancer Institute, Cairo University
  • Mohammed A. A. Elshaer Agricultural Biochemistry Department, Faculty of Agriculture, Al-Azhar University, Cairo, 11823, Egypt https://orcid.org/0000-0001-9110-6596
  • Shady Selim Department of Pesticide Chemistry and Technology, Faculty of Desert and Environmental Agriculture, Matrouh University, Egypt https://orcid.org/0000-0002-6971-914X

Keywords:

Anticancer, Antifungal activity, HepG2 cell line, Methanol extracts, Pruning tree residues, Phenolic compounds

Abstract

Methanol extracts (MEs) were obtained from pruning parts of some cultivated plants in Egypt, namely Mangifera indica (leaves), Moringa oleifera (seeds), Psidium guajava (leaves), and Citrus limon (branches). The chemical components present in the MEs were identified by HPLC. The cytotoxic activity of the four natural MEs was assessed for the HepG2 cell line after 24, 48, and 72 h of incubation using the sulforhodamine-B (SRB) assay. The antifungal effect was assessed against Aspergillus flavus and Aspergillus terreus. Based on HPLC, the MEs from M. indica leaf extract contained eugenol and ellagic acid; α-tocopherol, and p-coumaric acid in M. oleifera seeds; eugenol and caffeic acid in P. guava leaves; and ferulic acid and caffeic acid in C. limon branches. After 24 h of treatment, the EC50 for cell growth was 205 µg/mL from M. indica leaf ME. At 1000 µg/mL, M. oleifera seed ME, M. indica leaf ME, and C. limon branch ME showed the greatest fungal growth inhibition (FGI) percentages against A. flavus. At 1000 and 500 µg/mL M. oleifera, M. indica at 1000 µg/mL, and M. oleifera at 250 µg/mL, MEs exhibited the highest FGI% values against A. terreus.

Downloads

Published

2026-01-07 — Updated on 2026-01-08

How to Cite

Salem, M. Z. M., Al-Kubeisi, A. K., Ashmawy, A. M., Mansour, M. M. A., Aboelela, S., Elshaer, M. A. A., & Selim , S. (2026). Extracts from Some Tree Pruning Residues and their Cytotoxicity on the HepG2 Cell Line Using the Sulforhodamine-B Assay and their Antifungal Activity . BioResources, 21(1), 1603–1626. Retrieved from https://ojs.bioresources.com/index.php/BRJ/article/view/24795

Issue

Vol. 21 No. 1 (2026)

Section

Research Article or Brief Communication