Efficacy and Molecular Docking Study of Main Constituents of Murraya paniculata Biomass Extract Against Helicobacter pylori

Abstract

Natural compounds have received extra attention through the current decade to suppress Helicobacter pylori growth. This study investigated the phytochemical characterization of Murraya paniculata fruit extract (MPFE) and its estimation against different activities of H. pylori. Moreover, the molecular docking interactions (MDI) of catechin and kaempferol with H. pylori proteins were examined. Several compounds were detected via high performance liquid chromatography in MPFE with various concentrations. Of these, catechin, kaempferol, chlorogenic acid, and vanillin were measured as 11,000, 4960, 4610, and 65.8 µg/g, respectively. Excellent inhibition of H. pylori was recorded with an inhibition zone 24.3 mm using MPFE compared to the activity of standard antibiotic (16.2 mm). Both minimum inhibitory concentration and minimal bactericidal concentration (MBC) of MPFE were 60.5 µg/mL, whereas it was 15.6 µg/mL using standard antibiotic. The biofilm of H. pylori was inhibited by 25, 50, and 75% of MPFE MBC to a level of 68.2, 84.1, and 90.4%, respectively. Hemolysis caused by MPFE was prevented to a level of 21.2, 6.8, and, 3.3% at 25, 50, and 75% of MIC, respectively. The authors implemented the MDI using Molecular Operating Environment (MOE) software. The screened compounds interacted well with the H. pylori protein (PDB ID: 3K1H).