The Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes

Alkali Lignin on Laccase Activity and Mycelial Biomass

Authors

  • Mei-Ling Han College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China; Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang 065000, Hebei, China; Edible and Medicinal Fungi Research and Development Center of Universities/Colleges in Hebei Province, Langfang 065000, Hebei, China https://orcid.org/0000-0003-4936-3083
  • Yi-Lin Zhao College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China
  • Tian-Yu Liu College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China
  • Lu-Sen Bian Experimental Centre of Forestry in North China, Warm Temperate Zone Forestry Jiulong Mountain National Permanent Scientific Research Base, Chinese Academy of Forestry, Beijing 102300, China https://orcid.org/0000-0003-3660-9056
  • Zi-Han Li College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China
  • Ming-Xue Li College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China
  • Jia Li College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China
  • Xiao-Qi Yang College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China
  • Qi An College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China; Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang 065000, Hebei, China https://orcid.org/0000-0001-5917-2497

Keywords:

Flammulina velutipes, Laccase, Alkali lignin, Populus beijingensis, Mycelial biomass

Abstract

The effect of alkali lignin on laccase activity and mycelial biomass of Flammulina velutipes was investigated under different fermentation temperature. The secretion of laccase by F. velutipes has certain specificity. Specifically, laccase activity could not be detected by F. velutipes CCMSSC 00103 under most fermentation medium, but could be detected by F. velutipes FL 19 under more fermentation medium. Among the three strains of F. velutipes, the laccase secretion capacity of F. velutipes FL 19 was obviously better than that of F. velutipes CCMSSC 05331 and F. velutipes CCMSSC 00103. Maximum laccase activity with the value of 73.42 ± 4.74 U/L was secreted by F. velutipes FL 19 in fermentation medium 2 appeared at 2th day. The presence of alkali lignin was useful for improving laccase activity secreted by F. velutipes and the effect of alkali lignin on improving laccase activity exceeded that of Populus beijingensis. F. velutipes were more suitable for secreting laccase at 26 ℃ due to the maximum laccase activity measured at 26 ℃ (73.42 ± 4.74 U/L) higher than that measured at 33 ℃ (70.71 ± 6.89 U/L). The results were useful for selecting suitable medium and temperature of F. velutipes strains to produce low-cost laccase.

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Published

2025-01-29

How to Cite

Han, M.-L., Zhao, Y.-L., Liu, T.-Y., Bian, L.-S., Li, Z.-H., Li, M.-X., … An, Q. (2025). The Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes: Alkali Lignin on Laccase Activity and Mycelial Biomass. BioResources, 20(1), 2243–2258. Retrieved from https://ojs.bioresources.com/index.php/BRJ/article/view/24104

Issue

Vol. 20 No. 1 (2025)

Section

Research Article or Brief Communication