Andrographis paniculata Mediated AuNPs as Anti-Microbial, Antioxidant, Anti-Diabetic Agents and their Efficacy in Wound Healing of Mice

Abstract

Gold nanoparticles (AuNPs) are known to have low toxicity and biocompatibility. Meanwhile, Andrographis paniculata (A. paniculata) is a medicinal plant known for its therapeutic benefits. Traditional methods for the synthesis of AuNPs frequently depend on toxic compounds, which present environmental and health hazards. In this work, A. paniculata was combined with AuNPs to enhance its antimicrobial, antioxidant, and wound healing effects, thereby offering a safer and more effective alternative compared to current available methods. Water-soluble gold metal ions were reduced to neutral metal nanoparticles in the course of reaction with aqueous A. paniculata extract. The biosynthesised AuNPs was examined using UV-Vis, FTIR Spectroscopy, TEM, XRD, SAED Diffraction, EDX, DLS, and zeta potential measurements. AuNPs were incorporated into nonionic surfactant to form a thick, readily spreadable hydrogel. The mixture was found to enhance the wound contraction rate with no skin irritation observed in treated mice. The study demonstrated a simple and environmentally safe approach to produce AuNPs with improved antimicrobial, antioxidant, antidiabetic and wound healing abilities. The AuNPs-PF127 hydrogel is a non-toxic and bio-friendly delivery technology that shows potential in promoting healing of wounds, burns, etc.