Sustainable Utilization of Agave-Derived Sitosterol: A Review of Isolation Methods and Pharmacological Activities

Abstract

Agave species are increasingly recognized as promising sources of bioactive phytochemicals with therapeutic potential. Among these, β-sitosterol (BSS) and its glucoside (BSSG) have gained attention for their wound-healing, anti-inflammatory, antioxidant, and immunomodulatory properties. In vitro, these compounds enhance fibroblast viability and regulate cytokine production. In vivo, extracts from Agave angustifolia bagasse (BagEE), obtained through microwave-assisted extraction (MAE), significantly accelerate wound closure and re-epithelialization. MAE, particularly when combined with alkaline catalysts, yields higher concentrations of BSS and BSSG compared to conventional methods. However, despite its environmental and efficiency advantages, supercritical fluid extraction remains underutilized for isolating phytosterols from Agave. This review highlights interspecies variation in bioactive profiles, the critical impact of extraction methodology on compound yield and activity, and the potential for valorizing agro-industrial residues. These findings emphasize the value of Agave-derived sterols in the development of sustainable, plant-based therapeutics. Further research is needed to standardize extraction protocols, achieve comprehensive characterization of active metabolites, and evaluate their clinical efficacy—advancing innovation in bioproduct development aligned with circular economy principles.